Apoptotic Response to DNA Damage in Pluripotent Stem Cells Facilitates Pharmacologic Purging of Teratoma Forming Progenitor Cells

Technology #2012-090

Pluripotent stem cells have been the focus of bioengineering efforts designed to generate regenerative products, yet harnessing therapeutic capacity while minimizing the risk of dysregulated growth remains a formidable challenge. The risk of residual undifferentiated stem cells within differentiated progenitors requires a targeted approach to eliminate contaminating cells prior to in vivo delivery. We have validated a sensitive apoptotic response to DNA damaging agents within several types of pluripotent stem cells, which can be exploited to decrease the risk of dysregulated tumor growth in order to augment the therapeutic potential and safety profile of transplant-ready, bioengineered progenitor cells.