Jon H. Behringer
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2017-284 – Humanized PD-1, B7-H1 (PD-L1) and combination PD-1/B7-H1 (PD-L1) Knock-In Mouse Models Mayo Clinic researchers have produced humanized PD-1 and B7-H1 (PD-L1) mice using a homozygous “knock-in” strategy to meet the need for an in vivo model that can be used in the development of safe and effective drugs targeting PD-1 and B7-H1. The exons encoding the ligand binding extracellular Ig like... Read More
2016-372 – Mayo Clinic Researchers have discovered that microbiome associated protein 1 (MAP1)B1 is the antigen for PCA-2 autoantibodeis.
2017-235 – Mayo Clinic has discovered that novel STAT3 inhibitors would potentially be a therapeutic treatment in pediatric tumor Diffuse Intrinsic Pontine Glioma (DIPG). Novel STAT3 inhibitors selectively killed tumor cells found in DIPG.
2016-117 – Mayo Clinic has discovered that the use of USP7 inhibitors sensitize HER2+breast cancer cells to existing HER2+ therapies.
2015-066 – Mayo Clinic has discovered that USP10, an ubiquitin protease, deubinquinates AMPK and inhibits mTOR activity. Activating USP10 could have therapeutic effects in treating diabetes and cancer.
1998-039 – Through the use of gene targeting in mouse embryonic stem (ES) cells we have successfully generated a line of mice deficient in eosinophil granule major basic protein-1 (mMBP-1). These mice would be useful in drug discovery programs directed toward developing treatments for asthma, allergy, inflammation and a variety of eosinophil-related disorders. PMID: 11067904
2014-430 – Mouse model for human progeroid syndrome. Genotype/Phenotype: Sprtn hypomorphic, premature aging (progeria with humans). Utility: disease model for progeria. PMID: 25501849
2010-142 – Conditional NKAP knockout mouse. Genotype/Phenotype: NKAP is a transcriptional repressor of the Notch signaling pathway. The cre-lox system was used to remove exon 3 of NKAP and disrupt repressor function of NKAP. Loss of NKAP function early in T cell development resulted in a severe block in T cell development. Utility: Mouse model for examining NKAP function and the role of NKAP in T... Read More
2009-333 – ERB (estrogen receptor beta) mouse monoclonal antibody. Epitope: amino acids 1-140 Known Applications: Enzyme-linked immunosorbent assay (ELISA), Immunohistochemistry (IHC), Western blot (WB) Known Reactivity: Human
2009-216 – Mouse expressing human rab9 GTPase. Genotype/Phenotype: Mice demonstrate a dramatic reduction in storage of gangliosides and an approximately 22% increase in lifespan relative to controls. Utility: Useful for studies of secretion in the intestine and/or liver. Possible application to other lysosomal storage diseases. Model for in vivo studies of rab9 regulation and activity. PMID:... Read More
2007-110 – Genotype/Phenotype: Mice expressing human MUC1 (mucin) were mated with mice expressing elastase-driven SV40 T antigen. The resulting mice spontaneously develop pancreatic tumors that express high levels of the MUC1. Utility: Useful for investigating immune responses during tumor progression. PMID link: 10975866
2007-016 – Mouse expressing the human alpha-synuclein gene for the study of Parkinson’s disease. Genotype/Phenotype: These mice express the human alpha-synuclein gene, under the control of its endogenous human promoter and regulatory elements. Genomic multiplication of this locus in humans results in Parkinson’s disease with subsequent dementia, with post-mortem transitional or diffuse Lewy body... Read More
2006-220 – Hi52C is a mouse monoclonal antibody (IgG) generated against recombinant human FKBP52. The antibody is highly specific for FKBP52 and recognizes antigen on Western blots and will immunoprecipitate native protein complexes containing FKBP52. Hi52C is known to crossreact with FKBP52 from human, mouse, and rabbit sources. PMID Publication:15831525
2006-219 – Hi51B is a mouse monoclonal antibody (IgG) generated against recombinant human FKBP51. The antibody is highly specific for FKBP51 and recognizes antigen on Western blots and will immunoprecipitate native protein complexes containing FKBP51. Hi51B is known to crossreact with FKBP51 from human, mouse, and rabbit sources.
2006-116 – Mice expressing multiple human HLA class II molecules for the study of immune response to bacterial superantigens. Genotype/Phenotype: Mice express multiple varieties of human HLA class II molecules. Utility: Model systems for studying bacterial superantigens. PMID: 16428778 PMID: 18501769 PMID: 18086265
2004-287 – Rabbit polyclonal antibodies were generated to a specific phosphorylated peptide in the Smad3 protein. Epitope: COOH-GSPSIRCSpSVpS Known Applications: Western blot (WB) Known Reactivity: Human PMID 15520863
2003-128 – Human brain tumors that show invasive behavior in the brains of immunocompromised mice. Numerous, well-characterized tissue grafts are available.
2000-124 – Rat IgG Monoclonal Antibody Reactive Against Murine MBP Major basic protein, Clone 14.7.4 Known Applications: Immunohistochemistry (IHC), Western blot (WB) Known Reactivity: Mouse NIH publication 11067904
2000-020 – Mouse strain allowing inducible gene expression in the heart. Genotype/Phenotype: Utilizes a tetracycline- inducible cardiac-specific promoter (a-MHC) to drive a transactivator activating expression of any target gene. Utility: System for modulating gene expression in cardiac tissue. This technology uses the TET-ON/TET-OFF System®. PMID publication: 11437283
2013-180 – Snail is a protein that, when it is active induces an invasive phenotype in cancer cells. Inactivation of Snail can occur by phosphorylation of its amino acid residue serine 11 (see attached publication). We have generated a phosphosite specific antibody that targets the protein Snail, when it is phosphorylated at serine 11. This antibody can be utilized as a research tool, but also allows to... Read More
2013-102 – The Hdac3 conditional knockout mouse model made with Osx-Cre is a useful tool to study the molecular and physiological mechanisms by which factors (e.g., OPG and others) produced in the skeleton, particularly by immature osteoblasts and osteoblast progenitor cells, influence energy metabolism and prevent diseases like type 2 diabetes.
2013-082 – Antigen specific recognition is involved in adaptive immune responses. Most T lymphocytes express an antigen specific αβ T Cell Receptor (TCR) responsible for recognizing its ligand (peptide/MHC) and responding through various effector functions. In an attempt to better understand TCR function, extensive efforts have been placed in discerning the structural and biochemical makeup of the TCR.... Read More
2013-061 – We have developed an ongoing procedure at Mayo Clinic for obtaining and xenografting fresh pancreatic adenocarcinoma tumors into SCID mice. The tissues are obtained through an institutionally review board approved process, and informed consent is obtained from the patients. We have full annotation of clinical characteristics and disease course of the patients. Pretreatment blood samples... Read More
Genetically Engineered Mouse Model of Multiple Myeloma Without IoxP Sites (Vk*MYCwoLoxP) and Transplantable Cell Lines
2013-058 – A transgenic mouse model of multiple myeloma. Vk*MYCwoLoxP mice have conditional MYC activation in germinal center B cells. Mice progress to indolent myeloma with features highly characteristic of human disease. These mice can be used to study the biology and drug treatment of multiple myeloma, and can be crossed with conditional mice strains expressing Cre recombinase. Transplantable... Read More
2012-252 – Patient derived tumor tissue samples that have been demonstrated to grow in mice.
2012-123 – This mouse model contains TFPIFlox allele with LoxP sites flanking exon 4 of the TFPi gene. When bred to mice that express Cre recombinase, the resulting off spring will have the sequences encoding the Kuntz1 domain of the TFPI protein deleted in cre-expressing tissues. As the TFPI-K1 domain is necessary for TFPI inhibition of the tissue factor coagulation pathway, these mice may be useful... Read More
2012-122 – A transgenic mouse model having vascular smooth muscle cell directed overexpression of a mouse tissue factor pathway inhibitor cDNA sequence encoding the TFPI alpha isoform. These mice may be useful in studying the role of vascular TFPI expression in intravascular thrombosis, the tissue factor pathway in vascular disease, coagulation, innate immunity, angiogenesis and lipid metabolism.
2012-055 – Background Transcription factor STAT3 plays an active role in the initiation and proliferation of malignant brain tumors. Thus, small molecule STAT3 inhibitors have potential as therapeutic agents for malignant gliomas as well as other STAT3-dependent tumors. Technology Description Mayo Clinic Researchers have synthesized a series of novel compounds to block the STAT3 pathway. These... Read More
2012-045 – Normal rat cholangiocytes (NRCs) are intrahepatic bile duct epithelial cellls that have been developed as in vitro model to study the physiology and pathophysiology of these cells in culture. NRCs form a confluent polarized monolayer when grown on collagen-coated filters of tissue culture inserts with easy access to both apical and basolateral cell surfaces. NCRs display many... Read More
2010-320 – The MDQ-30 is a 28-item instrument in which the items query symptoms over the previous 30 days. Seventeen items use a dichotomous format, two items use a Likert scale, one item uses multiple non-hierarchical options, six items use multiple hierarchical options, and two items are multi-item dichotomous scales. MDQ-30 items follow a stem-and-leaf format and are divided into three symptom... Read More
2010-306 – Thyroid cancer cell lines were created from patient tumor tissues directly from the resected tumor tissue, tissue minced and cultured in cell culture media. These cells lines will be characterized at the molecular level [genetic defects, short tandem DNA repeat analysis (STR)] and phenotypically with regards to growth in cell culture and animals. These well characterized cell lines should... Read More
2010-305 – Cell lines were created from patient tumor tissues directly from the resected tumor tissue, tissue minced and cultured in cell culture media. These cells lines will be characterized at the molecular level (genetic defects, short tandem DNA repeat analysis (STR)) and phenotypically with regards to growth in cell culture and animals. These well characterized cell lines should prove to be useful... Read More
2010-206 – Patient derived tumor tissue samples that have been demonstrated to grow in mice.
2010-203 – Reagents to study the biology of the rare human bronchopulmonary carcinoid tumor are very limited. We have created cell lines derived from tumors resected from patients treated at Mayo Clinic. These cell lines will aid investigators to test existing and future medications for efficacy in killing these tumors on the cell lines prior to human studies. These cell lines will aid investigations... Read More
2009-211 – Technology Description Mayo Clinic researchers have discovered that USP10 is the missing link between autophagy and cancer formation. USP10 is a regulator of p53 degradation and activity. USP10 is stabilized upon DNA damage and deubiquitinates p53, reversing Mdm-2 induced nuclear export and degradation. USP10 suppresses tumor cell proliferation in cells expressing wild-type p53. USP10... Read More
2009-132 – A monoclonal antibody to MDC1, a key mediator of DNA damage response.
2008-277 – Anaplastic thyroid carcinoma (ATC) is one of the deadliest cancers with lethality of >99% and survival average of 4 months. Recent publications have demonstrated over 40% of thyroid cell lines are of questionable origin especially ATC cell lines (Schweppe et al., J Clin Endocrinol Metab 2008). We have developed four cell lines from human ATC tumor tissues. These cell lines represent the... Read More
2008-163 – These mice conditionally express the human tau cDNA (4RON isoform) containing the FTDP-17 mutation (P301L) in exon 10. These mice utilize the TET-ON/TET-OFF® system to express approximately 7X human tau in comparison to murine tau. Both the tTA transgene (Memory Pharmaceuticals) and the tau transgene (Mayo) must be present to yield expression of the human tau. Doxycycline in the diet of the... Read More
2008-016 – Four mouse anti-SINA/SIAH monoclonal antibodies are available for licensing. Details are as follows. Two monoclonal antibodies against the SINA-C-terminal peptide (C-GC-FDTSIAQLFADNGNLGINVTISLV) (a 27-mer): (1) 8G7H12, (2) 4B4B6. Two monoclonal antibodies against the SINA-N-terminal peptide (SNKINPKRREPTAA-GGC) (a 17-mer): (1) 24E6H3, (2) 22B9B5. References Rebecca L. Schmidt,... Read More
2007-330 – We created mice that contain human LRRK2 cDNA encoding either the wild-type or G2019S mutant form of the gene (hereafter termed “responder line(s).” The cDNA in these animals has been placed behind a minimal CMV promoter containing TetO and will only produce human LRRK2 protein when combined (in bigenic mice) with tTA. We have demonstrated in COS7 cells that the human LRRK2 expression from... Read More
2007-326 – JJ12 is a mouse monoclonal IgG antibody that was prepared against the human protein p23, a co-chaperone for the molecular chaperone protein Hsp90. p23 was isolated into balb/c mice, and hybridoma cells were made by fusion with the cell line P3NS-11-AG4-1(NS-1). JJ12 has a high affinity for p23 from humans and several other vertebrates. It is useful for the measurement of p23 by western... Read More
2007-325 – JJ6 is a mouse monoclonal IgG antibody that was prepared against the human protein p23, a co-chaperone for the molecular chaperone protein Hsp90. p23 was isolated and injected into balb/c mice, and hybridoma cells were made by fusion with the cell line P3NS-11-AG4-1 (NS-1). JJ6 has a high affinity for p23 from humans and several other vertebrates. It is useful for the measurement of p23 by... Read More
2007-323 – This is a monoclonal antibody against GCUNC45 (General Cell UNC45) also called SMAP-1 which has sequence similarity with the SMUNC45 (Striated muscle UNC45). These are homologues of the C. elegans UNC45. This antibody is useful western blotting and imunohistochemistry.
2007-288 – Mice in which the endogenous leucine-rich repeat kinase 2 gene (LRRK2) has been ablated. Exon 41 has been flanked with loxP sites to alow Cre-mediated deletion. The region deleted contains the serion/threonine protein kinase catalytic domain. Splicing of exon 40 to 42 causes a frameshift mutation with the introduction of an early stop codon (TGA).
2007-259 – We have generated 2 B-cell lines from 2 individual CVID patients who have a specific mutation in the TACI gene. TACI gene mutations are associated with 10-15% of CVID cases. The A181E mutation has been described as being significantly associated with CVID patients compared to normal controls. EBV-transformation of B-cells has been traditionally used to immortalize patient cells and provide a... Read More
2007-230 – We have replace the mouse islet amyloid polypeptide (IAPP, amylin) gene with the human IAPP gene. Human IAPP is readily converted to amyloid fibrils. Amyloids have been associated with a large number of human diseases that are associated with cell death. Mouse IAPP is non-amyloidogenic. By replacing the mouse IAPP with the human IAPP gene we have created a physiologic model of hman IAPP... Read More
2007-027 – Mice that over-express the human wild-type and mutant leucine-rich repeat kinase 2 gene (LRRK2).
2005-316 – These mice do not express the gene for CD229.
2005-247 – MAPT (tau) is a protein involved in regulating the structure and function of nerve cells. The clones contain DNA that encodes the tau protein and allow it to be made in cell lines.
Genetic Polymorphisms in the Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) gene in Caucasian, African American, Han Chinese and Mexican American Populations
2005-156 – Some of the known substrates of CYP19A1 (aromatase) include testosterone and androstenedione. It is expressed in the ovaries, testes, placenta, fetal liver, adipose tissue, chondrocytes, osteoblasts, vasculature smooth muscle, and brain. Increased or decreased enzyme activity is associated with several diseases including breast and endometrial cancer. Several novel polymorphisms have been... Read More