2015-151 – Several novel naturally processed and HLA presented influenza A/H5N1-derived peptides from a class I HLA-A0201 molecules have been identified. A mass spectrometry approach was used to identify five influenza peptides from a B-cell line (homozygous for HLA-A0201) after infection with influenza A/H5N1 virus. The immunologic relevance and specificity of these identified influenza-derived... Read More
"FACS-TB" a Novel Laboratory Method and Biomarker for Detection and Stratification of Latent Tuberculosis Infection
2013-109 – Accurate detection of the latent form of tuberculosis (TB) infection remains a major clinical and public health problem worldwide, which in the United States, disproportionally affects minorities, foreign-born individuals and patients with weak immune defenses (e.g. patients with HIV infection andlor on immunosuppressive medications). We designed and validated a new method that detects... Read More
2011-249 – TB-ELISPOT is an optimized Interferon-gamma release assay (IGRA) that accurately detects latent tuberculosis infection (LTBI). This novel TB-ELISPOT method incorporate research-grade technology to enhance antigen-specific activation of T-cells by utilizing co-stimulatory antibodies and best antigen stimulation conditions (48 hrs) in a multiparametric platform that compare specific M.... Read More
2009-189 – We have invented a technique for rapidly removing biofilms from the surface of explanted orthopaedic implants (e.g. prosthetic joints). The dislodged and disaggregated bacteria are suspended in solution and amenable to detection using culture or rapid detection strategies. This disclosure lists the bacteria that would need to be targeted to detect most cases of orthopaedic implant... Read More
2007-295 – To address difficulties in the identification of microsporidia from stool specimens in the clinical diagnostic laboratory, we developed two rapid, real-time PCR assays designed for the LightCycler instrument (Roche Applied Sciences). The LightCycler PCR (LC PCR) assay sensitivities were compared to that of the reference method, a routine smear with trichrome blue stain. Microsporidia DNA is... Read More
1995-102 – Detection with the use of broad range primers and the PCR assay for the detection of the WA-1 Agent.